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Quality Control for Genome-Wide Association Studies

Cedric Gondro, S H Lee, H K Lee, Laercio R Porto-Neto

Research output: Chapter in Book/Report/Conference proceedingChapterResearch

17 Citations (Scopus)

Abstract

This chapter overviews the quality control (QC) issues for SNP-based genotyping methods used in genome-wide association studies. The main metrics for evaluating the quality of the genotypes are discussed followed by a worked out example of QC pipeline starting with raw data and finishing with a fully filtered dataset ready for downstream analysis. The emphasis is on automation of data storage, filtering, and manipulation to ensure data integrity throughout the process and on how to extract a global summary from these high dimensional datasets to allow better-informed downstream analytical decisions. All examples will be run using the R statistical programming language followed by a practical example using a fully automated QC pipeline for the Illumina platform.
Original languageEnglish
Title of host publicationGenome-Wide Association Studies and Genomic Prediction
EditorsCedric Gondro, Julius van der Werf, Ben Hayes
Place of PublicationNew York, United States of America
PublisherHumana Press
Pages129-147
Edition1
ISBN (Print)9781627034463, 9781627034470
DOIs
Publication statusPublished - 2013

Publication series

NameMethods in Molecular Biology
Number1019
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Quantitative Genetics (incl Disease and Trait Mapping Genetics)

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