Microrna Profiling in Cattle Divergently Selected for Residual Feed Intake

Wijdan Al-Husseini; Cedric Gondro; Robert M Herd; John Gibson; P F Arthur; Yizhou Chen

Microrna Profiling in Cattle Divergently Selected for Residual Feed Intake

Wijdan Al-Husseini
, Cedric Gondro
, Robert M Herd
, John Gibson
, P F Arthur
, Yizhou Chen

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

MicroRNAs (miRNAs) are short non-coding RNAs that post-transcriptionally regulate expression of mRNAs in many biological pathways. Here we report comprehensive miRNA profiles by deep sequencing in Angus cattle divergently selected for residual feed intake (RFI). Two miRNA libraries were constructed from pooled RNA extracted from livers of low and high RFI cattle, and sequenced with the Illumina Genome Analyser. We identified 305 known bovine miRNAs. bta-miR-143, bta-miR-30, bta-miR-122, bta-miR-378 and bta-let-7 were the top 5 most abundant miRNA families expressed in liver, representing more than 63% of expressed miRNAs. Mir-143 is the most expressed bovine miRNA in liver, and is up-regulated in high RFI cattle. Mir-122 is the second most expressed miRNA in liver and is down regulated in high RFI animals. The differentially expressed miRNAs may play important roles in the regulation of the bioprocesses responsible for variation in RFI in cattle.

Original language	English
Title of host publication	Proceedings of the Association for the Advancement of Animal Breeding and Genetics
Editors	Nicolas Lopez Villalobos
Place of Publication	Armidale, Australia
Publisher	Association for the Advancement of Animal Breeding and Genetics (AAABG)
Pages	368-371
Volume	20
ISBN (Print)	9780473260569
Publication status	Published - 2013
Event	AAABG 2013: 20th Conference of the Association for the Advancement of Animal Breeding and Genetics: Translating Science into Action - Napier, New Zealand Duration: 20 Oct 2013 → 23 Oct 2013

Conference

Conference	AAABG 2013: 20th Conference of the Association for the Advancement of Animal Breeding and Genetics: Translating Science into Action
City	Napier, New Zealand
Period	20/10/13 → 23/10/13

Keywords

Gene Expression (incl Microarray and other genome-wide approaches)

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Al-Husseini, W., Gondro, C., Herd, R. M., Gibson, J., Arthur, P. F., & Chen, Y. (2013). Microrna Profiling in Cattle Divergently Selected for Residual Feed Intake. In N. Lopez Villalobos (Ed.), Proceedings of the Association for the Advancement of Animal Breeding and Genetics (Vol. 20, pp. 368-371). Association for the Advancement of Animal Breeding and Genetics (AAABG). https://hdl.handle.net/1959.11/14031

Al-Husseini, Wijdan ; Gondro, Cedric ; Herd, Robert M et al. / Microrna Profiling in Cattle Divergently Selected for Residual Feed Intake. Proceedings of the Association for the Advancement of Animal Breeding and Genetics. editor / Nicolas Lopez Villalobos. Vol. 20 Armidale, Australia : Association for the Advancement of Animal Breeding and Genetics (AAABG), 2013. pp. 368-371

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title = "Microrna Profiling in Cattle Divergently Selected for Residual Feed Intake",

abstract = "MicroRNAs (miRNAs) are short non-coding RNAs that post-transcriptionally regulate expression of mRNAs in many biological pathways. Here we report comprehensive miRNA profiles by deep sequencing in Angus cattle divergently selected for residual feed intake (RFI). Two miRNA libraries were constructed from pooled RNA extracted from livers of low and high RFI cattle, and sequenced with the Illumina Genome Analyser. We identified 305 known bovine miRNAs. bta-miR-143, bta-miR-30, bta-miR-122, bta-miR-378 and bta-let-7 were the top 5 most abundant miRNA families expressed in liver, representing more than 63\% of expressed miRNAs. Mir-143 is the most expressed bovine miRNA in liver, and is up-regulated in high RFI cattle. Mir-122 is the second most expressed miRNA in liver and is down regulated in high RFI animals. The differentially expressed miRNAs may play important roles in the regulation of the bioprocesses responsible for variation in RFI in cattle.",

keywords = "Gene Expression (incl Microarray and other genome-wide approaches)",

author = "Wijdan Al-Husseini and Cedric Gondro and Herd, \{Robert M\} and John Gibson and Arthur, \{P F\} and Yizhou Chen",

year = "2013",

language = "English",

isbn = "9780473260569",

volume = "20",

pages = "368--371",

editor = "\{Lopez Villalobos\}, Nicolas",

booktitle = "Proceedings of the Association for the Advancement of Animal Breeding and Genetics",

publisher = "Association for the Advancement of Animal Breeding and Genetics (AAABG)",

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Al-Husseini, W, Gondro, C, Herd, RM, Gibson, J, Arthur, PF & Chen, Y 2013, Microrna Profiling in Cattle Divergently Selected for Residual Feed Intake. in N Lopez Villalobos (ed.), Proceedings of the Association for the Advancement of Animal Breeding and Genetics. vol. 20, Association for the Advancement of Animal Breeding and Genetics (AAABG), Armidale, Australia, pp. 368-371, AAABG 2013: 20th Conference of the Association for the Advancement of Animal Breeding and Genetics: Translating Science into Action, Napier, New Zealand, 20/10/13. <https://hdl.handle.net/1959.11/14031>

Microrna Profiling in Cattle Divergently Selected for Residual Feed Intake. / Al-Husseini, Wijdan; Gondro, Cedric; Herd, Robert M et al.
Proceedings of the Association for the Advancement of Animal Breeding and Genetics. ed. / Nicolas Lopez Villalobos. Vol. 20 Armidale, Australia: Association for the Advancement of Animal Breeding and Genetics (AAABG), 2013. p. 368-371.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Microrna Profiling in Cattle Divergently Selected for Residual Feed Intake

AU - Al-Husseini, Wijdan

AU - Gondro, Cedric

AU - Herd, Robert M

AU - Gibson, John

AU - Arthur, P F

AU - Chen, Yizhou

PY - 2013

Y1 - 2013

N2 - MicroRNAs (miRNAs) are short non-coding RNAs that post-transcriptionally regulate expression of mRNAs in many biological pathways. Here we report comprehensive miRNA profiles by deep sequencing in Angus cattle divergently selected for residual feed intake (RFI). Two miRNA libraries were constructed from pooled RNA extracted from livers of low and high RFI cattle, and sequenced with the Illumina Genome Analyser. We identified 305 known bovine miRNAs. bta-miR-143, bta-miR-30, bta-miR-122, bta-miR-378 and bta-let-7 were the top 5 most abundant miRNA families expressed in liver, representing more than 63% of expressed miRNAs. Mir-143 is the most expressed bovine miRNA in liver, and is up-regulated in high RFI cattle. Mir-122 is the second most expressed miRNA in liver and is down regulated in high RFI animals. The differentially expressed miRNAs may play important roles in the regulation of the bioprocesses responsible for variation in RFI in cattle.

AB - MicroRNAs (miRNAs) are short non-coding RNAs that post-transcriptionally regulate expression of mRNAs in many biological pathways. Here we report comprehensive miRNA profiles by deep sequencing in Angus cattle divergently selected for residual feed intake (RFI). Two miRNA libraries were constructed from pooled RNA extracted from livers of low and high RFI cattle, and sequenced with the Illumina Genome Analyser. We identified 305 known bovine miRNAs. bta-miR-143, bta-miR-30, bta-miR-122, bta-miR-378 and bta-let-7 were the top 5 most abundant miRNA families expressed in liver, representing more than 63% of expressed miRNAs. Mir-143 is the most expressed bovine miRNA in liver, and is up-regulated in high RFI cattle. Mir-122 is the second most expressed miRNA in liver and is down regulated in high RFI animals. The differentially expressed miRNAs may play important roles in the regulation of the bioprocesses responsible for variation in RFI in cattle.

KW - Gene Expression (incl Microarray and other genome-wide approaches)

UR - http://www.aaabg.org/aaabghome/proceedings20.php

M3 - Conference contribution

SN - 9780473260569

VL - 20

SP - 368

EP - 371

BT - Proceedings of the Association for the Advancement of Animal Breeding and Genetics

A2 - Lopez Villalobos, Nicolas

PB - Association for the Advancement of Animal Breeding and Genetics (AAABG)

CY - Armidale, Australia

T2 - AAABG 2013: 20th Conference of the Association for the Advancement of Animal Breeding and Genetics: Translating Science into Action

Y2 - 20 October 2013 through 23 October 2013

ER -

Al-Husseini W, Gondro C, Herd RM, Gibson J, Arthur PF, Chen Y. Microrna Profiling in Cattle Divergently Selected for Residual Feed Intake. In Lopez Villalobos N, editor, Proceedings of the Association for the Advancement of Animal Breeding and Genetics. Vol. 20. Armidale, Australia: Association for the Advancement of Animal Breeding and Genetics (AAABG). 2013. p. 368-371

Microrna Profiling in Cattle Divergently Selected for Residual Feed Intake

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