Method for the Preparation of Mucosal Flaps from Jejunum of Laying Hens for Transporter Studies in Ussing Chambers

Isabelle Ruhnke; Ilen Roehe; Wilfried Meyer; Susan Kroeger; Konrad Neumann; Juergen Zentek

Method for the Preparation of Mucosal Flaps from Jejunum of Laying Hens for Transporter Studies in Ussing Chambers

Isabelle Ruhnke
, Ilen Roehe
, Wilfried Meyer
, Susan Kroeger
, Konrad Neumann
, Juergen Zentek

School of Environmental & Rural Science

Research output: Contribution to conference › Abstract

Abstract

Introduction: Ussing chambers are frequently used for in vitro-evaluation of intestinal transport physiology in many species. However, detailed information about the procedure of tissue preparation for investigating intestine from laying hens is not provided by the literature. The current study aimed to describe the Ussing chamber procedure for investigating the jejunal tissue from laying hens using a specific preparation method and to investigate the benefit of glutamine in the experimental buffer solution.

Original language	English
Publication status	Published - 2012
Event	ESVCN Congress 2012: 16th Congress of the European Society of Veterinary and Comparative Nutrition - Bydgoszcz, Poland Duration: 13 Sept 2012 → 15 Sept 2012

Conference

Conference	ESVCN Congress 2012: 16th Congress of the European Society of Veterinary and Comparative Nutrition
City	Bydgoszcz, Poland
Period	13/09/12 → 15/09/12

Keywords

Veterinary Diagnosis and Diagnostics

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https://hdl.handle.net/1959.11/20170

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title = "Method for the Preparation of Mucosal Flaps from Jejunum of Laying Hens for Transporter Studies in Ussing Chambers",

abstract = "Introduction: Ussing chambers are frequently used for in vitro-evaluation of intestinal transport physiology in many species. However, detailed information about the procedure of tissue preparation for investigating intestine from laying hens is not provided by the literature. The current study aimed to describe the Ussing chamber procedure for investigating the jejunal tissue from laying hens using a specific preparation method and to investigate the benefit of glutamine in the experimental buffer solution.",

keywords = "Veterinary Diagnosis and Diagnostics",

author = "Isabelle Ruhnke and Ilen Roehe and Wilfried Meyer and Susan Kroeger and Konrad Neumann and Juergen Zentek",

year = "2012",

language = "English",

note = "ESVCN Congress 2012: 16th Congress of the European Society of Veterinary and Comparative Nutrition ; Conference date: 13-09-2012 Through 15-09-2012",

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TY - CONF

T1 - Method for the Preparation of Mucosal Flaps from Jejunum of Laying Hens for Transporter Studies in Ussing Chambers

AU - Ruhnke, Isabelle

AU - Roehe, Ilen

AU - Meyer, Wilfried

AU - Kroeger, Susan

AU - Neumann, Konrad

AU - Zentek, Juergen

PY - 2012

Y1 - 2012

N2 - Introduction: Ussing chambers are frequently used for in vitro-evaluation of intestinal transport physiology in many species. However, detailed information about the procedure of tissue preparation for investigating intestine from laying hens is not provided by the literature. The current study aimed to describe the Ussing chamber procedure for investigating the jejunal tissue from laying hens using a specific preparation method and to investigate the benefit of glutamine in the experimental buffer solution.

AB - Introduction: Ussing chambers are frequently used for in vitro-evaluation of intestinal transport physiology in many species. However, detailed information about the procedure of tissue preparation for investigating intestine from laying hens is not provided by the literature. The current study aimed to describe the Ussing chamber procedure for investigating the jejunal tissue from laying hens using a specific preparation method and to investigate the benefit of glutamine in the experimental buffer solution.

KW - Veterinary Diagnosis and Diagnostics

UR - http://www.esvcn.eu/society/congress

M3 - Abstract

T2 - ESVCN Congress 2012: 16th Congress of the European Society of Veterinary and Comparative Nutrition

Y2 - 13 September 2012 through 15 September 2012

ER -

Method for the Preparation of Mucosal Flaps from Jejunum of Laying Hens for Transporter Studies in Ussing Chambers

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