Method analysis for a novel trait: comparing locations for haemoglobin sampling in piglets

Haemoglobin (Hb) testing on farm is a new technology within the pig industry that can be used for improving survival in piglets (Rootwelt et al., 2012) and as a selection criterion for iron content in pork (Hermesch and Jones, 2012). The testing requires a 10-μL sample of blood to be collected from the piglet. The most logical time to collect this sample is at litter processing when piglets are already being handled, thereby minimising stress on the piglet. The aims of the study were to determine first whether the location from which the blood sample was collected from the piglet (ear versus tail) influenced Hb measurement, and second, whether the order of collection (ear or tail first) had any impact on Hb measurement. The sow of the litter was also included as an effect. It was hypothesised that the ear would be the optimal location for blood collection and that it would not have an effect on the Hb levels.