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Measuring Intracellular hsp70 in Leukocytes by Flow Cytometry

Linda Agnew

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Heat shock or stress proteins are constitutively expressed redox-sensitive proteins, the synthesis of which is induced in almost all organisms exposed to a range of stressors, including heat shock, oxidative stress, free radicals, UV radiation, and heavy metals. This unit details a method, with supporting protocols, for the measurement of their expression in peripheral blood leukocytes by flow cytometry.
Original languageEnglish
Pages (from-to)2.21.1-2.21.12
JournalCurrent Protocols in Toxicology
Volume49
Issue number1
DOIs
Publication statusPublished - Aug 2011

Keywords

  • Toxicology (incl Clinical Toxicology)
  • Cellular Immunology

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