Isolation of Marek's Disease virus from dust samples from commercial chicken farms

Sheikh Zahid Hussian; Afm Fakhrul Islam; Susan Burgess; Paul Scott Reynolds; Steve William Walkden-Brown

Isolation of Marek's Disease virus from dust samples from commercial chicken farms

Sheikh Zahid Hussian
, Afm Fakhrul Islam
, Susan Burgess
, Paul Scott Reynolds
, Steve William Walkden-Brown

Research output: Contribution to journal › Conference article › peer-review

Abstract

Marek's disease virus (MDV) replicates in chicken feather follicle epithelium and these cells serve as a source of infection for other chickens. We report the use of chicken dust collected in the field to isolate MDV in specific pathogen-free chickens. Chickens were infected via the respiratory route with MDV-positive chicken dust. Quantitative polymerase chain reaction (q-PCR) assay was used to quantify MDV in dust and spleen samples from the experiment. Two out of five field dust samples successfully induced infections with subsequent MDV recovery. Serological testing of chickens at day 56 post infection indicated that no other major poultry pathogens were transmitted by the infective dust material. Use of poultry dust as primary material for isolating MDV offers potential advantages over fresh tissues and this work has shown that with some refinement it is a feasible approach.

Original language	English
Pages (from-to)	100-104
Journal	Proceedings of the Australian Poultry Science Symposium
Volume	17
Publication status	Published - 2005
Event	APSS 2005: 17th Annual Australian Poultry Science Symposium - Sydney, Australia Duration: 7 Feb 2005 → 9 Feb 2005

Keywords

Veterinary Virology

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@article{2e5117f71af64e39a6e37615e751b096,

title = "Isolation of Marek's Disease virus from dust samples from commercial chicken farms",

abstract = "Marek's disease virus (MDV) replicates in chicken feather follicle epithelium and these cells serve as a source of infection for other chickens. We report the use of chicken dust collected in the field to isolate MDV in specific pathogen-free chickens. Chickens were infected via the respiratory route with MDV-positive chicken dust. Quantitative polymerase chain reaction (q-PCR) assay was used to quantify MDV in dust and spleen samples from the experiment. Two out of five field dust samples successfully induced infections with subsequent MDV recovery. Serological testing of chickens at day 56 post infection indicated that no other major poultry pathogens were transmitted by the infective dust material. Use of poultry dust as primary material for isolating MDV offers potential advantages over fresh tissues and this work has shown that with some refinement it is a feasible approach.",

keywords = "Veterinary Virology",

author = "Hussian, \{Sheikh Zahid\} and Islam, \{Afm Fakhrul\} and Susan Burgess and Reynolds, \{Paul Scott\} and Walkden-Brown, \{Steve William\}",

year = "2005",

language = "English",

volume = "17",

pages = "100--104",

journal = "Proceedings of the Australian Poultry Science Symposium",

issn = "1034-3466",

publisher = "University of Sydney",

note = "APSS 2005: 17th Annual Australian Poultry Science Symposium ; Conference date: 07-02-2005 Through 09-02-2005",

}

TY - JOUR

T1 - Isolation of Marek's Disease virus from dust samples from commercial chicken farms

AU - Hussian, Sheikh Zahid

AU - Islam, Afm Fakhrul

AU - Burgess, Susan

AU - Reynolds, Paul Scott

AU - Walkden-Brown, Steve William

PY - 2005

Y1 - 2005

N2 - Marek's disease virus (MDV) replicates in chicken feather follicle epithelium and these cells serve as a source of infection for other chickens. We report the use of chicken dust collected in the field to isolate MDV in specific pathogen-free chickens. Chickens were infected via the respiratory route with MDV-positive chicken dust. Quantitative polymerase chain reaction (q-PCR) assay was used to quantify MDV in dust and spleen samples from the experiment. Two out of five field dust samples successfully induced infections with subsequent MDV recovery. Serological testing of chickens at day 56 post infection indicated that no other major poultry pathogens were transmitted by the infective dust material. Use of poultry dust as primary material for isolating MDV offers potential advantages over fresh tissues and this work has shown that with some refinement it is a feasible approach.

AB - Marek's disease virus (MDV) replicates in chicken feather follicle epithelium and these cells serve as a source of infection for other chickens. We report the use of chicken dust collected in the field to isolate MDV in specific pathogen-free chickens. Chickens were infected via the respiratory route with MDV-positive chicken dust. Quantitative polymerase chain reaction (q-PCR) assay was used to quantify MDV in dust and spleen samples from the experiment. Two out of five field dust samples successfully induced infections with subsequent MDV recovery. Serological testing of chickens at day 56 post infection indicated that no other major poultry pathogens were transmitted by the infective dust material. Use of poultry dust as primary material for isolating MDV offers potential advantages over fresh tissues and this work has shown that with some refinement it is a feasible approach.

KW - Veterinary Virology

UR - http://trove.nla.gov.au/work/6382329?selectedversion=NBD7527026

UR - http://www.vetsci.usyd.edu.au/apss/documents/2005/APSS2005-hussain-pp100-104.pdf

M3 - Conference article

SN - 1034-3466

VL - 17

SP - 100

EP - 104

JO - Proceedings of the Australian Poultry Science Symposium

JF - Proceedings of the Australian Poultry Science Symposium

T2 - APSS 2005: 17th Annual Australian Poultry Science Symposium

Y2 - 7 February 2005 through 9 February 2005

ER -

Isolation of Marek's Disease virus from dust samples from commercial chicken farms

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