'In vitro' acclimatisation of Rosa sp cv. Red cascade as a function of gradual reduction in relative humidity

Majid Danesh; Acram Taji

'In vitro' acclimatisation of Rosa sp cv. Red cascade as a function of gradual reduction in relative humidity

Majid Danesh
, Acram Taji

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

Acclimatisation as the final stage in micropropagation was investigated owing to its importance in the survival of 'in vitro' grown plants, Differences between the 'in vitro' and 'ex vitro' environment result in physiological and morphological abnormalities, reflecting the way in which plantlets adapt to in vitro conditions. Variations in relative humidity, light intensity and sucrose concentration in 'in vitro' grown Rosa sp. cv. Red cascade were examined in relation to wax content, stomatal density, chlorophyll content and consequent plantlets survival rate. In conjunction with low sucrose concentration, a gradual reduction in relative humidity 'in vitro' to 86% resulted in a 100% survival rate of plantlets directly transferred into pots. Reducing relative humidity during the rooting stage reduced acclimatisation duration. Survival rate was decreased as the light intensity decreased. Overall results indicate that cultured plantlets can resemble greenhouse-grown plants under modified environmental conditions, thus increasing the economic potential of large-scale, commercial tissue culture ventures.

Original language	English
Title of host publication	The Importance of Plant Tissue Culture and Biotechnology in Plant Sciences - Proceedings of the 7th International Association for Plant Tissue Culture Biotechnology (The Australian Branch) Conference
Editors	Acram Taji, Richard Williams
Place of Publication	Armidale, Australia
Publisher	University of New England
Pages	263-272
ISBN (Print)	186389781X
Publication status	Published - 2002
Event	IAPTC Australian Branch 2002: 7th International Congress of Plant Tissue and Cell Culture Australian Branch conference - University of New England, Armidale, Australia Duration: 20 Jan 2002 → 23 Jan 2002

Conference

Conference	IAPTC Australian Branch 2002: 7th International Congress of Plant Tissue and Cell Culture Australian Branch conference
City	Armidale, Australia
Period	20/01/02 → 23/01/02

Keywords

Genetically Modified Horticulture Plants

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https://hdl.handle.net/1959.11/3974

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Danesh, M., & Taji, A. (2002). 'In vitro' acclimatisation of Rosa sp cv. Red cascade as a function of gradual reduction in relative humidity. In A. Taji, & R. Williams (Eds.), The Importance of Plant Tissue Culture and Biotechnology in Plant Sciences - Proceedings of the 7th International Association for Plant Tissue Culture Biotechnology (The Australian Branch) Conference (pp. 263-272). University of New England. https://hdl.handle.net/1959.11/3974

Danesh, Majid ; Taji, Acram. / 'In vitro' acclimatisation of Rosa sp cv. Red cascade as a function of gradual reduction in relative humidity. The Importance of Plant Tissue Culture and Biotechnology in Plant Sciences - Proceedings of the 7th International Association for Plant Tissue Culture Biotechnology (The Australian Branch) Conference. editor / Acram Taji ; Richard Williams. Armidale, Australia : University of New England, 2002. pp. 263-272

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title = "'In vitro' acclimatisation of Rosa sp cv. Red cascade as a function of gradual reduction in relative humidity",

abstract = "Acclimatisation as the final stage in micropropagation was investigated owing to its importance in the survival of 'in vitro' grown plants, Differences between the 'in vitro' and 'ex vitro' environment result in physiological and morphological abnormalities, reflecting the way in which plantlets adapt to in vitro conditions. Variations in relative humidity, light intensity and sucrose concentration in 'in vitro' grown Rosa sp. cv. Red cascade were examined in relation to wax content, stomatal density, chlorophyll content and consequent plantlets survival rate. In conjunction with low sucrose concentration, a gradual reduction in relative humidity 'in vitro' to 86\% resulted in a 100\% survival rate of plantlets directly transferred into pots. Reducing relative humidity during the rooting stage reduced acclimatisation duration. Survival rate was decreased as the light intensity decreased. Overall results indicate that cultured plantlets can resemble greenhouse-grown plants under modified environmental conditions, thus increasing the economic potential of large-scale, commercial tissue culture ventures.",

keywords = "Genetically Modified Horticulture Plants",

author = "Majid Danesh and Acram Taji",

year = "2002",

language = "English",

isbn = "186389781X",

pages = "263--272",

editor = "Acram Taji and Richard Williams",

booktitle = "The Importance of Plant Tissue Culture and Biotechnology in Plant Sciences - Proceedings of the 7th International Association for Plant Tissue Culture Biotechnology (The Australian Branch) Conference",

publisher = "University of New England",

note = "IAPTC Australian Branch 2002: 7th International Congress of Plant Tissue and Cell Culture Australian Branch conference ; Conference date: 20-01-2002 Through 23-01-2002",

}

Danesh, M & Taji, A 2002, 'In vitro' acclimatisation of Rosa sp cv. Red cascade as a function of gradual reduction in relative humidity. in A Taji & R Williams (eds), The Importance of Plant Tissue Culture and Biotechnology in Plant Sciences - Proceedings of the 7th International Association for Plant Tissue Culture Biotechnology (The Australian Branch) Conference. University of New England, Armidale, Australia, pp. 263-272, IAPTC Australian Branch 2002: 7th International Congress of Plant Tissue and Cell Culture Australian Branch conference, Armidale, Australia, 20/01/02. <https://hdl.handle.net/1959.11/3974>

'In vitro' acclimatisation of Rosa sp cv. Red cascade as a function of gradual reduction in relative humidity. / Danesh, Majid; Taji, Acram.
The Importance of Plant Tissue Culture and Biotechnology in Plant Sciences - Proceedings of the 7th International Association for Plant Tissue Culture Biotechnology (The Australian Branch) Conference. ed. / Acram Taji; Richard Williams. Armidale, Australia: University of New England, 2002. p. 263-272.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - 'In vitro' acclimatisation of Rosa sp cv. Red cascade as a function of gradual reduction in relative humidity

AU - Danesh, Majid

AU - Taji, Acram

PY - 2002

Y1 - 2002

N2 - Acclimatisation as the final stage in micropropagation was investigated owing to its importance in the survival of 'in vitro' grown plants, Differences between the 'in vitro' and 'ex vitro' environment result in physiological and morphological abnormalities, reflecting the way in which plantlets adapt to in vitro conditions. Variations in relative humidity, light intensity and sucrose concentration in 'in vitro' grown Rosa sp. cv. Red cascade were examined in relation to wax content, stomatal density, chlorophyll content and consequent plantlets survival rate. In conjunction with low sucrose concentration, a gradual reduction in relative humidity 'in vitro' to 86% resulted in a 100% survival rate of plantlets directly transferred into pots. Reducing relative humidity during the rooting stage reduced acclimatisation duration. Survival rate was decreased as the light intensity decreased. Overall results indicate that cultured plantlets can resemble greenhouse-grown plants under modified environmental conditions, thus increasing the economic potential of large-scale, commercial tissue culture ventures.

AB - Acclimatisation as the final stage in micropropagation was investigated owing to its importance in the survival of 'in vitro' grown plants, Differences between the 'in vitro' and 'ex vitro' environment result in physiological and morphological abnormalities, reflecting the way in which plantlets adapt to in vitro conditions. Variations in relative humidity, light intensity and sucrose concentration in 'in vitro' grown Rosa sp. cv. Red cascade were examined in relation to wax content, stomatal density, chlorophyll content and consequent plantlets survival rate. In conjunction with low sucrose concentration, a gradual reduction in relative humidity 'in vitro' to 86% resulted in a 100% survival rate of plantlets directly transferred into pots. Reducing relative humidity during the rooting stage reduced acclimatisation duration. Survival rate was decreased as the light intensity decreased. Overall results indicate that cultured plantlets can resemble greenhouse-grown plants under modified environmental conditions, thus increasing the economic potential of large-scale, commercial tissue culture ventures.

KW - Genetically Modified Horticulture Plants

UR - http://trove.nla.gov.au/work/22270294?selectedversion=NBD24389715

M3 - Conference contribution

SN - 186389781X

SP - 263

EP - 272

BT - The Importance of Plant Tissue Culture and Biotechnology in Plant Sciences - Proceedings of the 7th International Association for Plant Tissue Culture Biotechnology (The Australian Branch) Conference

A2 - Taji, Acram

A2 - Williams, Richard

PB - University of New England

CY - Armidale, Australia

T2 - IAPTC Australian Branch 2002: 7th International Congress of Plant Tissue and Cell Culture Australian Branch conference

Y2 - 20 January 2002 through 23 January 2002

ER -

Danesh M, Taji A. 'In vitro' acclimatisation of Rosa sp cv. Red cascade as a function of gradual reduction in relative humidity. In Taji A, Williams R, editors, The Importance of Plant Tissue Culture and Biotechnology in Plant Sciences - Proceedings of the 7th International Association for Plant Tissue Culture Biotechnology (The Australian Branch) Conference. Armidale, Australia: University of New England. 2002. p. 263-272

'In vitro' acclimatisation of Rosa sp cv. Red cascade as a function of gradual reduction in relative humidity

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