Gene expression profiling as a tool for understanding the host response to cattle ticks

Emily K Piper; Nicholas N Jonsson; Constantin Constantinoiu; Cedric Gondro; Ala E Lew-Tabor; Megan E Vance; Louise A Jackson

Gene expression profiling as a tool for understanding the host response to cattle ticks

Emily K Piper, Nicholas N Jonsson, Constantin Constantinoiu, Cedric Gondro, Ala E Lew-Tabor, Megan E Vance, Louise A Jackson

School of Environmental & Rural Science

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

The cattle tick, 'Rhipicephalus Boophilus microplus' (formerly 'Boophilus microplus'), is the most significant parasite of cattle in Australia and threatens cattle production in tropical and subtropical countries worldwide. Some cattle breeds of mainly 'Bos indicus' origin develop a strong resistance to infestation with 'R. B. microplus', while other breeds of mainly 'Bos taurus' origin will succumb to anaemia and 'tick worry' in tick-infested pastures. Composite cattle ('B. indicus' x 'B. taurus') can have very wide ranges of tick resistance (Utech et al., 1978). Resistance to cattle tick infestation is primarily manifest against the larval stage and results in the immature tick failing to make a successful attachment and obtain a meal (Seifert, 1971). It is widely accepted that resistance to tick infestation in cattle is immunologically mediated and involves both cell-mediated and humoral immune responses. Previous research has demonstrated a role for the hypersensitivity response in the rejection of larvae from resistant 'B. taurus' cattle. Gene expression profiling using microarrays and quantitative PCR (qPCR) are popular tools to complement more classical measurements of immune reactivity and function. The immune response to 'R. B. microplus' infestation in 'B. indicus' and 'B. indicus' x 'B. taurus' cattle has not been studied in great detail. Using a combination of gene expression tools, together with measurements of immune system function such as histology and ELISA, we aimed to describe those responses of highly tick-resistant cattle that differ from those of tick-susceptible cattle.

Original language	English
Title of host publication	Proceedings of Advances in the Knowledge of Parasite Resistance of Ruminant Hosts and Parasites
Editors	Luciana Correia de Almeida Regitano, Simone Cristina Meo Niciura, Ana Carolina de Souza Chagas, Marcia Cristina de Sena Oliveira, Marcelo Beltrao Molento, Mauricio de Alvarenga Mudadu, Luiz Lehmann Coutinho, Alessandro Francisco Talamini do Amarante
Place of Publication	Sao Carlos, Brazil
Publisher	Embrapa Southeastern Region Animal Husbandry
Pages	30-33
ISBN (Print)	9788586764233
Publication status	Published - 2011
Event	Advances in the Knowledge of Parasite Resistance of Ruminant Hosts and Parasites - Sao Carlos, Brazil Duration: 26 Sept 2011 → 7 Oct 2011

Conference

Conference	Advances in the Knowledge of Parasite Resistance of Ruminant Hosts and Parasites
City	Sao Carlos, Brazil
Period	26/09/11 → 7/10/11

Keywords

Gene Expression (incl Microarray and other genome-wide approaches)

Access to Document

https://hdl.handle.net/1959.11/12487

Handle.net

Persistent link

Cite this

Piper, E. K., Jonsson, N. N., Constantinoiu, C., Gondro, C., Lew-Tabor, A. E., Vance, M. E., & Jackson, L. A. (2011). Gene expression profiling as a tool for understanding the host response to cattle ticks. In L. Correia de Almeida Regitano, S. Cristina Meo Niciura, A. Carolina de Souza Chagas, M. Cristina de Sena Oliveira, M. Beltrao Molento, M. de Alvarenga Mudadu, L. Lehmann Coutinho, & A. Francisco Talamini do Amarante (Eds.), Proceedings of Advances in the Knowledge of Parasite Resistance of Ruminant Hosts and Parasites (pp. 30-33). Embrapa Southeastern Region Animal Husbandry. https://hdl.handle.net/1959.11/12487

Piper, Emily K ; Jonsson, Nicholas N ; Constantinoiu, Constantin et al. / Gene expression profiling as a tool for understanding the host response to cattle ticks. Proceedings of Advances in the Knowledge of Parasite Resistance of Ruminant Hosts and Parasites. editor / Luciana Correia de Almeida Regitano ; Simone Cristina Meo Niciura ; Ana Carolina de Souza Chagas ; Marcia Cristina de Sena Oliveira ; Marcelo Beltrao Molento ; Mauricio de Alvarenga Mudadu ; Luiz Lehmann Coutinho ; Alessandro Francisco Talamini do Amarante. Sao Carlos, Brazil : Embrapa Southeastern Region Animal Husbandry, 2011. pp. 30-33

@inproceedings{9b595af5d4714b78bf65fb729dd0ed8f,

title = "Gene expression profiling as a tool for understanding the host response to cattle ticks",

abstract = "The cattle tick, 'Rhipicephalus Boophilus microplus' (formerly 'Boophilus microplus'), is the most significant parasite of cattle in Australia and threatens cattle production in tropical and subtropical countries worldwide. Some cattle breeds of mainly 'Bos indicus' origin develop a strong resistance to infestation with 'R. B. microplus', while other breeds of mainly 'Bos taurus' origin will succumb to anaemia and 'tick worry' in tick-infested pastures. Composite cattle ('B. indicus' x 'B. taurus') can have very wide ranges of tick resistance (Utech et al., 1978). Resistance to cattle tick infestation is primarily manifest against the larval stage and results in the immature tick failing to make a successful attachment and obtain a meal (Seifert, 1971). It is widely accepted that resistance to tick infestation in cattle is immunologically mediated and involves both cell-mediated and humoral immune responses. Previous research has demonstrated a role for the hypersensitivity response in the rejection of larvae from resistant 'B. taurus' cattle. Gene expression profiling using microarrays and quantitative PCR (qPCR) are popular tools to complement more classical measurements of immune reactivity and function. The immune response to 'R. B. microplus' infestation in 'B. indicus' and 'B. indicus' x 'B. taurus' cattle has not been studied in great detail. Using a combination of gene expression tools, together with measurements of immune system function such as histology and ELISA, we aimed to describe those responses of highly tick-resistant cattle that differ from those of tick-susceptible cattle.",

keywords = "Gene Expression (incl Microarray and other genome-wide approaches)",

author = "Piper, \{Emily K\} and Jonsson, \{Nicholas N\} and Constantin Constantinoiu and Cedric Gondro and Lew-Tabor, \{Ala E\} and Vance, \{Megan E\} and Jackson, \{Louise A\}",

year = "2011",

language = "English",

isbn = "9788586764233",

pages = "30--33",

editor = "\{Correia de Almeida Regitano\}, Luciana and \{Cristina Meo Niciura\}, Simone and \{Carolina de Souza Chagas\}, Ana and \{Cristina de Sena Oliveira\}, Marcia and \{Beltrao Molento\}, Marcelo and \{de Alvarenga Mudadu\}, Mauricio and \{Lehmann Coutinho\}, Luiz and \{Francisco Talamini do Amarante\}, Alessandro",

booktitle = "Proceedings of Advances in the Knowledge of Parasite Resistance of Ruminant Hosts and Parasites",

publisher = "Embrapa Southeastern Region Animal Husbandry",

note = "Advances in the Knowledge of Parasite Resistance of Ruminant Hosts and Parasites ; Conference date: 26-09-2011 Through 07-10-2011",

}

Piper, EK, Jonsson, NN, Constantinoiu, C, Gondro, C, Lew-Tabor, AE, Vance, ME & Jackson, LA 2011, Gene expression profiling as a tool for understanding the host response to cattle ticks. in L Correia de Almeida Regitano, S Cristina Meo Niciura, A Carolina de Souza Chagas, M Cristina de Sena Oliveira, M Beltrao Molento, M de Alvarenga Mudadu, L Lehmann Coutinho & A Francisco Talamini do Amarante (eds), Proceedings of Advances in the Knowledge of Parasite Resistance of Ruminant Hosts and Parasites. Embrapa Southeastern Region Animal Husbandry, Sao Carlos, Brazil, pp. 30-33, Advances in the Knowledge of Parasite Resistance of Ruminant Hosts and Parasites, Sao Carlos, Brazil, 26/09/11. <https://hdl.handle.net/1959.11/12487>

Gene expression profiling as a tool for understanding the host response to cattle ticks. / Piper, Emily K; Jonsson, Nicholas N; Constantinoiu, Constantin et al.
Proceedings of Advances in the Knowledge of Parasite Resistance of Ruminant Hosts and Parasites. ed. / Luciana Correia de Almeida Regitano; Simone Cristina Meo Niciura; Ana Carolina de Souza Chagas; Marcia Cristina de Sena Oliveira; Marcelo Beltrao Molento; Mauricio de Alvarenga Mudadu; Luiz Lehmann Coutinho; Alessandro Francisco Talamini do Amarante. Sao Carlos, Brazil: Embrapa Southeastern Region Animal Husbandry, 2011. p. 30-33.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Gene expression profiling as a tool for understanding the host response to cattle ticks

AU - Piper, Emily K

AU - Jonsson, Nicholas N

AU - Constantinoiu, Constantin

AU - Gondro, Cedric

AU - Lew-Tabor, Ala E

AU - Vance, Megan E

AU - Jackson, Louise A

PY - 2011

Y1 - 2011

N2 - The cattle tick, 'Rhipicephalus Boophilus microplus' (formerly 'Boophilus microplus'), is the most significant parasite of cattle in Australia and threatens cattle production in tropical and subtropical countries worldwide. Some cattle breeds of mainly 'Bos indicus' origin develop a strong resistance to infestation with 'R. B. microplus', while other breeds of mainly 'Bos taurus' origin will succumb to anaemia and 'tick worry' in tick-infested pastures. Composite cattle ('B. indicus' x 'B. taurus') can have very wide ranges of tick resistance (Utech et al., 1978). Resistance to cattle tick infestation is primarily manifest against the larval stage and results in the immature tick failing to make a successful attachment and obtain a meal (Seifert, 1971). It is widely accepted that resistance to tick infestation in cattle is immunologically mediated and involves both cell-mediated and humoral immune responses. Previous research has demonstrated a role for the hypersensitivity response in the rejection of larvae from resistant 'B. taurus' cattle. Gene expression profiling using microarrays and quantitative PCR (qPCR) are popular tools to complement more classical measurements of immune reactivity and function. The immune response to 'R. B. microplus' infestation in 'B. indicus' and 'B. indicus' x 'B. taurus' cattle has not been studied in great detail. Using a combination of gene expression tools, together with measurements of immune system function such as histology and ELISA, we aimed to describe those responses of highly tick-resistant cattle that differ from those of tick-susceptible cattle.

AB - The cattle tick, 'Rhipicephalus Boophilus microplus' (formerly 'Boophilus microplus'), is the most significant parasite of cattle in Australia and threatens cattle production in tropical and subtropical countries worldwide. Some cattle breeds of mainly 'Bos indicus' origin develop a strong resistance to infestation with 'R. B. microplus', while other breeds of mainly 'Bos taurus' origin will succumb to anaemia and 'tick worry' in tick-infested pastures. Composite cattle ('B. indicus' x 'B. taurus') can have very wide ranges of tick resistance (Utech et al., 1978). Resistance to cattle tick infestation is primarily manifest against the larval stage and results in the immature tick failing to make a successful attachment and obtain a meal (Seifert, 1971). It is widely accepted that resistance to tick infestation in cattle is immunologically mediated and involves both cell-mediated and humoral immune responses. Previous research has demonstrated a role for the hypersensitivity response in the rejection of larvae from resistant 'B. taurus' cattle. Gene expression profiling using microarrays and quantitative PCR (qPCR) are popular tools to complement more classical measurements of immune reactivity and function. The immune response to 'R. B. microplus' infestation in 'B. indicus' and 'B. indicus' x 'B. taurus' cattle has not been studied in great detail. Using a combination of gene expression tools, together with measurements of immune system function such as histology and ELISA, we aimed to describe those responses of highly tick-resistant cattle that differ from those of tick-susceptible cattle.

KW - Gene Expression (incl Microarray and other genome-wide approaches)

M3 - Conference contribution

SN - 9788586764233

SP - 30

EP - 33

BT - Proceedings of Advances in the Knowledge of Parasite Resistance of Ruminant Hosts and Parasites

A2 - Correia de Almeida Regitano, Luciana

A2 - Cristina Meo Niciura, Simone

A2 - Carolina de Souza Chagas, Ana

A2 - Cristina de Sena Oliveira, Marcia

A2 - Beltrao Molento, Marcelo

A2 - de Alvarenga Mudadu, Mauricio

A2 - Lehmann Coutinho, Luiz

A2 - Francisco Talamini do Amarante, Alessandro

PB - Embrapa Southeastern Region Animal Husbandry

CY - Sao Carlos, Brazil

T2 - Advances in the Knowledge of Parasite Resistance of Ruminant Hosts and Parasites

Y2 - 26 September 2011 through 7 October 2011

ER -

Piper EK, Jonsson NN, Constantinoiu C, Gondro C, Lew-Tabor AE, Vance ME et al. Gene expression profiling as a tool for understanding the host response to cattle ticks. In Correia de Almeida Regitano L, Cristina Meo Niciura S, Carolina de Souza Chagas A, Cristina de Sena Oliveira M, Beltrao Molento M, de Alvarenga Mudadu M, Lehmann Coutinho L, Francisco Talamini do Amarante A, editors, Proceedings of Advances in the Knowledge of Parasite Resistance of Ruminant Hosts and Parasites. Sao Carlos, Brazil: Embrapa Southeastern Region Animal Husbandry. 2011. p. 30-33

Gene expression profiling as a tool for understanding the host response to cattle ticks

Abstract

Conference

Keywords

Access to Document

Handle.net

Fingerprint

Cite this