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Ganglion Cell Assessment in Rodents with Retinal Degeneration

  • Erica L Fletcher
  • , Ursula Greferath
  • , Susmita Saha
  • , Emily E Anderson
  • , Kirstan A Vessey

Research output: Chapter in Book/Report/Conference proceedingChapterResearchpeer-review

2 Citations (Scopus)

Abstract

Analysis of how retinal ganglion cells change in retinal degeneration is critical for evaluating the potential of photoreceptor restorative therapies. Immunocytochemistry in combination with image analysis provides a way for quantifying not only the density of ganglion cells during disease, but also information about their morphology and an evaluation of excitatory and inhibitory synaptic inputs. Here, we describe how indirect immunofluorescence can be used in retinal whole mounts to obtain information about ganglion cells in retinal degeneration.

Original languageEnglish
Title of host publicationMouse Retinal Phenotyping: Methods and Protocols
EditorsNaoyuki Tanimoto
Place of PublicationUnited States of America
PublisherHumana Press, Inc
Pages261-273
ISBN (Print)9781493977208, 9781493977192
DOIs
Publication statusPublished - 31 Dec 2018

Publication series

NameMethods in Molecular Biology
Number1753
ISSN (Print)1940-6029
ISSN (Electronic)1064-3745

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