Effect of O-glycosylation and tyrosinesulfation of leech-derived peptides on binding and inhibitory activity against thrombin

Yves S Y Hsieh; Deni Taleski; Brendan Wilkinson; Lakshmi C Wijeyewickrema; Ty E Adams; Robert N Pike; Richard J Payne

doi:10.1039/c1cc14773k

Effect of O-glycosylation and tyrosinesulfation of leech-derived peptides on binding and inhibitory activity against thrombin

Yves S Y Hsieh
, Deni Taleski
, Brendan Wilkinson
, Lakshmi C Wijeyewickrema
, Ty E Adams
, Robert N Pike
, Richard J Payne

Chemistry

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

Synthesis of sulfated and unsulfated (glyco)peptide fragments of Hirudin P6 (a potent anticoagulant from the leech 'Hirudinaria manillensis') is described. The effect of O-glycosylation and tyrosine sulfation on thrombin binding and peptidolytic activity was investigated, together with the inhibition of fibrinogen cleavage.

Original language	English
Pages (from-to)	1547-1549
Journal	Chemical Communications
Volume	48
Issue number	10
DOIs	https://doi.org/10.1039/c1cc14773k
Publication status	Published - 2012

Keywords

Organic Chemical Synthesis
Proteins and Peptides
Biologically Active Molecules

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10.1039/c1cc14773k

https://hdl.handle.net/1959.11/20595

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title = "Effect of O-glycosylation and tyrosinesulfation of leech-derived peptides on binding and inhibitory activity against thrombin",

abstract = "Synthesis of sulfated and unsulfated (glyco)peptide fragments of Hirudin P6 (a potent anticoagulant from the leech 'Hirudinaria manillensis') is described. The effect of O-glycosylation and tyrosine sulfation on thrombin binding and peptidolytic activity was investigated, together with the inhibition of fibrinogen cleavage.",

keywords = "Organic Chemical Synthesis, Proteins and Peptides, Biologically Active Molecules",

author = "Hsieh, \{Yves S Y\} and Deni Taleski and Brendan Wilkinson and Wijeyewickrema, \{Lakshmi C\} and Adams, \{Ty E\} and Pike, \{Robert N\} and Payne, \{Richard J\}",

year = "2012",

doi = "10.1039/c1cc14773k",

language = "English",

volume = "48",

pages = "1547--1549",

journal = "Chemical Communications",

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publisher = "Royal Society of Chemistry",

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TY - JOUR

T1 - Effect of O-glycosylation and tyrosinesulfation of leech-derived peptides on binding and inhibitory activity against thrombin

AU - Hsieh, Yves S Y

AU - Taleski, Deni

AU - Wilkinson, Brendan

AU - Wijeyewickrema, Lakshmi C

AU - Adams, Ty E

AU - Pike, Robert N

AU - Payne, Richard J

PY - 2012

Y1 - 2012

N2 - Synthesis of sulfated and unsulfated (glyco)peptide fragments of Hirudin P6 (a potent anticoagulant from the leech 'Hirudinaria manillensis') is described. The effect of O-glycosylation and tyrosine sulfation on thrombin binding and peptidolytic activity was investigated, together with the inhibition of fibrinogen cleavage.

AB - Synthesis of sulfated and unsulfated (glyco)peptide fragments of Hirudin P6 (a potent anticoagulant from the leech 'Hirudinaria manillensis') is described. The effect of O-glycosylation and tyrosine sulfation on thrombin binding and peptidolytic activity was investigated, together with the inhibition of fibrinogen cleavage.

KW - Organic Chemical Synthesis

KW - Proteins and Peptides

KW - Biologically Active Molecules

U2 - 10.1039/c1cc14773k

DO - 10.1039/c1cc14773k

M3 - Article

SN - 1359-7345

VL - 48

SP - 1547

EP - 1549

JO - Chemical Communications

JF - Chemical Communications

IS - 10

ER -

Effect of O-glycosylation and tyrosinesulfation of leech-derived peptides on binding and inhibitory activity against thrombin

Abstract

Keywords

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