Effect of ageing and oxidative stress on the glutathione concentration in different kidney regions

Noor Riyadh Thiab; Graham L Jones; Nicola King

Abstract

Oxidative stress has been implicated in ageing and the pathogenesis of chronic kidney disease. Glutathione (GSH) is the major intracellular thiol in the kidney and an indicator of this organ's redox status. The aim of this study was to investigate how the GSH concentration is affected by age and oxidative stress in different kidney regions. Kidneys were dissected from Male Wistar rats of 5, 12, 36 and 60 weeks-old. Slices of superficial cortex, outer or inner medulla were incubated for 30 min ± 0.2 mM H₂O₂ prior to homogenisation and centrifugation. GSH concentrations were measured colormetrically using a kit. Data are presented in nmol/mg protein, are means ± SE of n = 5 and statistical comparisons were carried out using ANOVA with an appropriate post-test. In all regions and all conditions the GSH concentration showed a similar pattern with 12 weeks-old > 36 weeks-old > 60 weeks-old and 5 weeks-old. The greatest concentration was measured in the 12 weeks-old with the superficial cortex (1509.16 ± 27.34) significantly greater than outer (851.67 ± 47.39, p

Original language	English
Pages	587-588
Publication status	Published - 2013
Event	13th International Congress on Amino Acids, Peptides and Proteins - Galveston, United States of America Duration: 5 Oct 2013 → 7 Oct 2013

Conference

Conference	13th International Congress on Amino Acids, Peptides and Proteins
City	Galveston, United States of America
Period	5/10/13 → 7/10/13

Keywords

Biochemistry and Cell Biology

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https://hdl.handle.net/1959.11/15091

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Cite this

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title = "Effect of ageing and oxidative stress on the glutathione concentration in different kidney regions",

abstract = "Oxidative stress has been implicated in ageing and the pathogenesis of chronic kidney disease. Glutathione (GSH) is the major intracellular thiol in the kidney and an indicator of this organ's redox status. The aim of this study was to investigate how the GSH concentration is affected by age and oxidative stress in different kidney regions. Kidneys were dissected from Male Wistar rats of 5, 12, 36 and 60 weeks-old. Slices of superficial cortex, outer or inner medulla were incubated for 30 min ± 0.2 mM H₂O₂ prior to homogenisation and centrifugation. GSH concentrations were measured colormetrically using a kit. Data are presented in nmol/mg protein, are means ± SE of n = 5 and statistical comparisons were carried out using ANOVA with an appropriate post-test. In all regions and all conditions the GSH concentration showed a similar pattern with 12 weeks-old > 36 weeks-old > 60 weeks-old and 5 weeks-old. The greatest concentration was measured in the 12 weeks-old with the superficial cortex (1509.16 ± 27.34) significantly greater than outer (851.67 ± 47.39, p",

keywords = "Biochemistry and Cell Biology",

author = "Thiab, \{Noor Riyadh\} and Jones, \{Graham L\} and Nicola King",

year = "2013",

language = "English",

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note = "13th International Congress on Amino Acids, Peptides and Proteins ; Conference date: 05-10-2013 Through 07-10-2013",

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TY - CONF

T1 - Effect of ageing and oxidative stress on the glutathione concentration in different kidney regions

AU - Thiab, Noor Riyadh

AU - Jones, Graham L

AU - King, Nicola

PY - 2013

Y1 - 2013

N2 - Oxidative stress has been implicated in ageing and the pathogenesis of chronic kidney disease. Glutathione (GSH) is the major intracellular thiol in the kidney and an indicator of this organ's redox status. The aim of this study was to investigate how the GSH concentration is affected by age and oxidative stress in different kidney regions. Kidneys were dissected from Male Wistar rats of 5, 12, 36 and 60 weeks-old. Slices of superficial cortex, outer or inner medulla were incubated for 30 min ± 0.2 mM H₂O₂ prior to homogenisation and centrifugation. GSH concentrations were measured colormetrically using a kit. Data are presented in nmol/mg protein, are means ± SE of n = 5 and statistical comparisons were carried out using ANOVA with an appropriate post-test. In all regions and all conditions the GSH concentration showed a similar pattern with 12 weeks-old > 36 weeks-old > 60 weeks-old and 5 weeks-old. The greatest concentration was measured in the 12 weeks-old with the superficial cortex (1509.16 ± 27.34) significantly greater than outer (851.67 ± 47.39, p

AB - Oxidative stress has been implicated in ageing and the pathogenesis of chronic kidney disease. Glutathione (GSH) is the major intracellular thiol in the kidney and an indicator of this organ's redox status. The aim of this study was to investigate how the GSH concentration is affected by age and oxidative stress in different kidney regions. Kidneys were dissected from Male Wistar rats of 5, 12, 36 and 60 weeks-old. Slices of superficial cortex, outer or inner medulla were incubated for 30 min ± 0.2 mM H₂O₂ prior to homogenisation and centrifugation. GSH concentrations were measured colormetrically using a kit. Data are presented in nmol/mg protein, are means ± SE of n = 5 and statistical comparisons were carried out using ANOVA with an appropriate post-test. In all regions and all conditions the GSH concentration showed a similar pattern with 12 weeks-old > 36 weeks-old > 60 weeks-old and 5 weeks-old. The greatest concentration was measured in the 12 weeks-old with the superficial cortex (1509.16 ± 27.34) significantly greater than outer (851.67 ± 47.39, p

KW - Biochemistry and Cell Biology

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T2 - 13th International Congress on Amino Acids, Peptides and Proteins

Y2 - 5 October 2013 through 7 October 2013

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