CYP21 as a candidate gene for androstenone on boar taint

P Arasta; Y Chen; Yuandan Zhang; Richard John Kerr; Hans Ulrich Graser; B Luxford; C Moran

CYP21 as a candidate gene for androstenone on boar taint

P Arasta
, Y Chen
, Yuandan Zhang
, Richard John Kerr
, Hans Ulrich Graser
, B Luxford
, C Moran

Animal Genetics and Breeding Unit

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

The Cytochrome P450 steroid, 21 Hydroxylase (CYP21), is a positional candidate gene for boar taint caused by an excessive level of androstenone mainly in fat and muscle. CYP21 contains 9 introns and 10 exons and is located in the swine lymphocyte antigen (SLA) region on chromosome 7. Sequencing of four sires from an Australian mapping pedigree has revealed 36 SNPs. 22 of these are in non-coding regions, and those in coding regions do not cause changes in the amino acid sequence of the protein. However, a SNP at position 2329 (intron 7) could influence the use of a theoretical alternative splice site. Quantitative analysis has shown that segregation of this SNP in the sire families has no impact on androstenone levels. RT-PCR and sequencing have provided no evidence of the use of this alternative splice site in testis RNA. CYP21 can be excluded as the locus responsible for the SSC7 androstenone QTL.

Original language	English
Title of host publication	Proceedings of the Seventeenth Conference for the Advancement of Animal Breeding and Genetics
Editors	AAABG: Association for the Advancement of Animal Breeding, Genetics
Place of Publication	Armidale, Australia
Publisher	Association for the Advancement of Animal Breeding and Genetics (AAABG)
Pages	65-68
Volume	17
ISBN (Print)	1921208139
Publication status	Published - 2007
Event	AAABG 2007: 17th Conference of the Association for the Advancement of Animal Breeding and Genetics - University of New England, Armidale, Australia Duration: 23 Sept 2007 → 26 Sept 2007

Conference

Conference	AAABG 2007: 17th Conference of the Association for the Advancement of Animal Breeding and Genetics
City	Armidale, Australia
Period	23/09/07 → 26/09/07

Keywords

Molecular Evolution

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https://hdl.handle.net/1959.11/4151

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Arasta, P., Chen, Y., Zhang, Y., Kerr, R. J., Graser, H. U., Luxford, B., & Moran, C. (2007). CYP21 as a candidate gene for androstenone on boar taint. In AAABG. Association for the Advancement of Animal Breeding, & Genetics (Eds.), Proceedings of the Seventeenth Conference for the Advancement of Animal Breeding and Genetics (Vol. 17, pp. 65-68). Association for the Advancement of Animal Breeding and Genetics (AAABG). https://hdl.handle.net/1959.11/4151

Arasta, P ; Chen, Y ; Zhang, Yuandan et al. / CYP21 as a candidate gene for androstenone on boar taint. Proceedings of the Seventeenth Conference for the Advancement of Animal Breeding and Genetics. editor / AAABG: Association for the Advancement of Animal Breeding ; Genetics. Vol. 17 Armidale, Australia : Association for the Advancement of Animal Breeding and Genetics (AAABG), 2007. pp. 65-68

@inproceedings{a8940b3170534d918ee16b7628cad1d0,

title = "CYP21 as a candidate gene for androstenone on boar taint",

abstract = "The Cytochrome P450 steroid, 21 Hydroxylase (CYP21), is a positional candidate gene for boar taint caused by an excessive level of androstenone mainly in fat and muscle. CYP21 contains 9 introns and 10 exons and is located in the swine lymphocyte antigen (SLA) region on chromosome 7. Sequencing of four sires from an Australian mapping pedigree has revealed 36 SNPs. 22 of these are in non-coding regions, and those in coding regions do not cause changes in the amino acid sequence of the protein. However, a SNP at position 2329 (intron 7) could influence the use of a theoretical alternative splice site. Quantitative analysis has shown that segregation of this SNP in the sire families has no impact on androstenone levels. RT-PCR and sequencing have provided no evidence of the use of this alternative splice site in testis RNA. CYP21 can be excluded as the locus responsible for the SSC7 androstenone QTL.",

keywords = "Molecular Evolution",

author = "P Arasta and Y Chen and Yuandan Zhang and Kerr, \{Richard John\} and Graser, \{Hans Ulrich\} and B Luxford and C Moran",

year = "2007",

language = "English",

isbn = "1921208139",

volume = "17",

pages = "65--68",

editor = "\{Association for the Advancement of Animal Breeding\}, AAABG: and Genetics",

booktitle = "Proceedings of the Seventeenth Conference for the Advancement of Animal Breeding and Genetics",

publisher = "Association for the Advancement of Animal Breeding and Genetics (AAABG)",

note = "AAABG 2007: 17th Conference of the Association for the Advancement of Animal Breeding and Genetics ; Conference date: 23-09-2007 Through 26-09-2007",

}

Arasta, P, Chen, Y, Zhang, Y, Kerr, RJ, Graser, HU, Luxford, B & Moran, C 2007, CYP21 as a candidate gene for androstenone on boar taint. in AAABG Association for the Advancement of Animal Breeding & Genetics (eds), Proceedings of the Seventeenth Conference for the Advancement of Animal Breeding and Genetics. vol. 17, Association for the Advancement of Animal Breeding and Genetics (AAABG), Armidale, Australia, pp. 65-68, AAABG 2007: 17th Conference of the Association for the Advancement of Animal Breeding and Genetics, Armidale, Australia, 23/09/07. <https://hdl.handle.net/1959.11/4151>

CYP21 as a candidate gene for androstenone on boar taint. / Arasta, P; Chen, Y; Zhang, Yuandan et al.
Proceedings of the Seventeenth Conference for the Advancement of Animal Breeding and Genetics. ed. / AAABG: Association for the Advancement of Animal Breeding; Genetics. Vol. 17 Armidale, Australia: Association for the Advancement of Animal Breeding and Genetics (AAABG), 2007. p. 65-68.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - CYP21 as a candidate gene for androstenone on boar taint

AU - Arasta, P

AU - Chen, Y

AU - Zhang, Yuandan

AU - Kerr, Richard John

AU - Graser, Hans Ulrich

AU - Luxford, B

AU - Moran, C

PY - 2007

Y1 - 2007

N2 - The Cytochrome P450 steroid, 21 Hydroxylase (CYP21), is a positional candidate gene for boar taint caused by an excessive level of androstenone mainly in fat and muscle. CYP21 contains 9 introns and 10 exons and is located in the swine lymphocyte antigen (SLA) region on chromosome 7. Sequencing of four sires from an Australian mapping pedigree has revealed 36 SNPs. 22 of these are in non-coding regions, and those in coding regions do not cause changes in the amino acid sequence of the protein. However, a SNP at position 2329 (intron 7) could influence the use of a theoretical alternative splice site. Quantitative analysis has shown that segregation of this SNP in the sire families has no impact on androstenone levels. RT-PCR and sequencing have provided no evidence of the use of this alternative splice site in testis RNA. CYP21 can be excluded as the locus responsible for the SSC7 androstenone QTL.

AB - The Cytochrome P450 steroid, 21 Hydroxylase (CYP21), is a positional candidate gene for boar taint caused by an excessive level of androstenone mainly in fat and muscle. CYP21 contains 9 introns and 10 exons and is located in the swine lymphocyte antigen (SLA) region on chromosome 7. Sequencing of four sires from an Australian mapping pedigree has revealed 36 SNPs. 22 of these are in non-coding regions, and those in coding regions do not cause changes in the amino acid sequence of the protein. However, a SNP at position 2329 (intron 7) could influence the use of a theoretical alternative splice site. Quantitative analysis has shown that segregation of this SNP in the sire families has no impact on androstenone levels. RT-PCR and sequencing have provided no evidence of the use of this alternative splice site in testis RNA. CYP21 can be excluded as the locus responsible for the SSC7 androstenone QTL.

KW - Molecular Evolution

UR - http://www.aaabg.org/livestocklibrary/2007/arasta065.pdf

UR - http://trove.nla.gov.au/work/35062558?selectedversion=NBD42373479

M3 - Conference contribution

SN - 1921208139

VL - 17

SP - 65

EP - 68

BT - Proceedings of the Seventeenth Conference for the Advancement of Animal Breeding and Genetics

A2 - Association for the Advancement of Animal Breeding, AAABG:

A2 - Genetics, null

PB - Association for the Advancement of Animal Breeding and Genetics (AAABG)

CY - Armidale, Australia

T2 - AAABG 2007: 17th Conference of the Association for the Advancement of Animal Breeding and Genetics

Y2 - 23 September 2007 through 26 September 2007

ER -

Arasta P, Chen Y, Zhang Y, Kerr RJ, Graser HU, Luxford B et al. CYP21 as a candidate gene for androstenone on boar taint. In Association for the Advancement of Animal Breeding AAABG, Genetics, editors, Proceedings of the Seventeenth Conference for the Advancement of Animal Breeding and Genetics. Vol. 17. Armidale, Australia: Association for the Advancement of Animal Breeding and Genetics (AAABG). 2007. p. 65-68

CYP21 as a candidate gene for androstenone on boar taint

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Keywords

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