APTEC: aptamer-tethered enzyme capture as a novel rapid diagnostic test for malaria

Roderick M Dirkzwager; Andrew B Kinghorn; Jack S Richards; Julian A Tanner

doi:10.1039/c5cc00438a

APTEC: aptamer-tethered enzyme capture as a novel rapid diagnostic test for malaria

Roderick M Dirkzwager
, Andrew B Kinghorn
, Jack S Richards
, Julian A Tanner

Animal Science

Research output: Contribution to journal › Article › peer-review

54 Citations (Scopus)

Abstract

We report the rapid diagnosis of malaria by aptamer-tethered enzyme capture (APTEC) whereby an aptamer captures biomarker Plasmodium falciparum lactate dehydrogenase (PfLDH) then activity is measured colorimetrically. The robust test was sensitive (limit of detection = 4.9 ng mL⁻¹) and could reliably diagnose malaria in clinical blood samples.

Original language	English
Pages (from-to)	4697-4700
Journal	Chemical Communications
Volume	51
Issue number	22
DOIs	https://doi.org/10.1039/c5cc00438a
Publication status	Published - 18 Mar 2015

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title = "APTEC: aptamer-tethered enzyme capture as a novel rapid diagnostic test for malaria",

abstract = "We report the rapid diagnosis of malaria by aptamer-tethered enzyme capture (APTEC) whereby an aptamer captures biomarker Plasmodium falciparum lactate dehydrogenase (PfLDH) then activity is measured colorimetrically. The robust test was sensitive (limit of detection = 4.9 ng mL−1) and could reliably diagnose malaria in clinical blood samples.",

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AU - Kinghorn, Andrew B

AU - Richards, Jack S

AU - Tanner, Julian A

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AB - We report the rapid diagnosis of malaria by aptamer-tethered enzyme capture (APTEC) whereby an aptamer captures biomarker Plasmodium falciparum lactate dehydrogenase (PfLDH) then activity is measured colorimetrically. The robust test was sensitive (limit of detection = 4.9 ng mL−1) and could reliably diagnose malaria in clinical blood samples.

U2 - 10.1039/c5cc00438a

DO - 10.1039/c5cc00438a

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JF - Chemical Communications

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ER -

APTEC: aptamer-tethered enzyme capture as a novel rapid diagnostic test for malaria

Abstract

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