Absolute quantification of Marek's disease virus serotype 2 (MDV2) using real-time polymerase chain reaction and its application to field dust samples

Katrin Renz; Brian Frederick Cheetham; Aminul Islam; Steve William Walkden-Brown

Absolute quantification of Marek's disease virus serotype 2 (MDV2) using real-time polymerase chain reaction and its application to field dust samples

Katrin Renz
, Brian Frederick Cheetham
, Aminul Islam
, Steve William Walkden-Brown

Research output: Contribution to journal › Conference article › peer-review

Abstract

Methods for taqman real-time PCR assays to detect the three serotypes of MDV are available (Islam et al., 2004), and an absolute quantification has been developed for MDV serotype 1 and serotype 3. The development of a method for absolute quantification of Marek's disease virus serotype 2 (MDV2) is described in this paper. Thus, it is now possible to perform qPCR assays for all three serotypes ofMDV on a sample. Absolute quantification of MDV2 in dust samples from poultry farms across Australia in a preliminary study, revealed the presence of MDV2 in 13 of 30 samples tested.

Original language	English
Pages (from-to)	153-156
Journal	Proceedings of the Australian Poultry Science Symposium
Volume	18
Publication status	Published - 2006
Event	APSS 2006: 18th Annual Australian Poultry Science Symposium - Sydney, Australia Duration: 20 Feb 2006 → 22 Feb 2006

Keywords

Virology

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@article{c9456ef68cb545e4b481abc11bd2b23f,

title = "Absolute quantification of Marek's disease virus serotype 2 (MDV2) using real-time polymerase chain reaction and its application to field dust samples",

abstract = "Methods for taqman real-time PCR assays to detect the three serotypes of MDV are available (Islam et al., 2004), and an absolute quantification has been developed for MDV serotype 1 and serotype 3. The development of a method for absolute quantification of Marek's disease virus serotype 2 (MDV2) is described in this paper. Thus, it is now possible to perform qPCR assays for all three serotypes ofMDV on a sample. Absolute quantification of MDV2 in dust samples from poultry farms across Australia in a preliminary study, revealed the presence of MDV2 in 13 of 30 samples tested.",

keywords = "Virology",

author = "Katrin Renz and Cheetham, \{Brian Frederick\} and Aminul Islam and Walkden-Brown, \{Steve William\}",

year = "2006",

language = "English",

volume = "18",

pages = "153--156",

journal = "Proceedings of the Australian Poultry Science Symposium",

issn = "1034-6260",

publisher = "University of Sydney",

note = "APSS 2006: 18th Annual Australian Poultry Science Symposium ; Conference date: 20-02-2006 Through 22-02-2006",

}

Absolute quantification of Marek's disease virus serotype 2 (MDV2) using real-time polymerase chain reaction and its application to field dust samples. / Renz, Katrin; Cheetham, Brian Frederick; Islam, Aminul et al.
In: Proceedings of the Australian Poultry Science Symposium, Vol. 18, 2006, p. 153-156.

Research output: Contribution to journal › Conference article › peer-review

TY - JOUR

T1 - Absolute quantification of Marek's disease virus serotype 2 (MDV2) using real-time polymerase chain reaction and its application to field dust samples

AU - Renz, Katrin

AU - Cheetham, Brian Frederick

AU - Islam, Aminul

AU - Walkden-Brown, Steve William

PY - 2006

Y1 - 2006

N2 - Methods for taqman real-time PCR assays to detect the three serotypes of MDV are available (Islam et al., 2004), and an absolute quantification has been developed for MDV serotype 1 and serotype 3. The development of a method for absolute quantification of Marek's disease virus serotype 2 (MDV2) is described in this paper. Thus, it is now possible to perform qPCR assays for all three serotypes ofMDV on a sample. Absolute quantification of MDV2 in dust samples from poultry farms across Australia in a preliminary study, revealed the presence of MDV2 in 13 of 30 samples tested.

AB - Methods for taqman real-time PCR assays to detect the three serotypes of MDV are available (Islam et al., 2004), and an absolute quantification has been developed for MDV serotype 1 and serotype 3. The development of a method for absolute quantification of Marek's disease virus serotype 2 (MDV2) is described in this paper. Thus, it is now possible to perform qPCR assays for all three serotypes ofMDV on a sample. Absolute quantification of MDV2 in dust samples from poultry farms across Australia in a preliminary study, revealed the presence of MDV2 in 13 of 30 samples tested.

KW - Virology

UR - http://www.vetsci.usyd.edu.au/apss/documents/2006/APSS2006-renz-pp153-156.pdf

UR - http://trove.nla.gov.au/work/6382329?selectedversion=NBD7527026

M3 - Conference article

SN - 1034-6260

VL - 18

SP - 153

EP - 156

JO - Proceedings of the Australian Poultry Science Symposium

JF - Proceedings of the Australian Poultry Science Symposium

T2 - APSS 2006: 18th Annual Australian Poultry Science Symposium

Y2 - 20 February 2006 through 22 February 2006

ER -

Absolute quantification of Marek's disease virus serotype 2 (MDV2) using real-time polymerase chain reaction and its application to field dust samples

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