TY - JOUR
T1 - A nuclear phylogenomic study of the angiosperm order Myrtales, exploring the potential and limitations of the universal Angiosperms353 probe set
AU - Maurin, Olivier
AU - Anest, Artemis
AU - Bellot, Sidonie
AU - Biffin, Edward
AU - Brewer, Grace
AU - Charles-Dominique, Tristan
AU - Cowan, Robyn S
AU - Dodsworth, Steven
AU - Epitawalage, Niroshini
AU - Gallego, Berta
AU - Giaretta, Augusto
AU - Goldenberg, Renato
AU - Gonçalves, Deise J P
AU - Graham, Shirley
AU - Hoch, Peter
AU - Mazine, Fiorella
AU - Low, Yee Wen
AU - McGinnie, Catherine
AU - Michelangeli, Fabián A
AU - Morris, Sarah
AU - Penneys, Darin S
AU - Pérez Escobar, Oscar Alejandro
AU - Pillon, Yohan
AU - Pokorny, Lisa
AU - Shimizu, Gustavo
AU - Staggemeier, Vanessa G
AU - Thornhill, Andrew H
AU - Tomlinson, Kyle W
AU - Turner, Ian M
AU - Vasconcelos, Thais
AU - Wilson, Peter G
AU - Zuntini, Alexandre R
AU - Baker, William J
AU - Forest, Félix
AU - Lucas, Eve
PY - 2021/7/31
Y1 - 2021/7/31
N2 - PREMISE: To further advance the understanding of the species- rich, economically and ecologically important angiosperm order Myrtales in the rosid clade, comprising nine families, approximately 400 genera and almost 14,000 species occurring on all continents (except Antarctica), we tested the Angiosperms353 probe kit.METHODS: We combined high- throughput sequencing and target enrichment with the Angiosperms353 probe kit to evaluate a sample of 485 species across 305 genera (76% of all genera in the order).RESULTS: Results provide the most comprehensive phylogenetic hypothesis for the order to date. Relationships at all ranks, such as the relationship of the early-diverging families, often reflect previous studies, but gene conflict is evident, and relationships previously found to be uncertain often remain so. Technical considerations for processing HTS data are also discussed.CONCLUSIONS: High- throughput sequencing and the Angiosperms353 probe kit are powerful tools for phylogenomic analysis, but better understanding of the genetic data available is required to identify genes and gene trees that account for likely incomplete lineage sorting and/or hybridization events.
AB - PREMISE: To further advance the understanding of the species- rich, economically and ecologically important angiosperm order Myrtales in the rosid clade, comprising nine families, approximately 400 genera and almost 14,000 species occurring on all continents (except Antarctica), we tested the Angiosperms353 probe kit.METHODS: We combined high- throughput sequencing and target enrichment with the Angiosperms353 probe kit to evaluate a sample of 485 species across 305 genera (76% of all genera in the order).RESULTS: Results provide the most comprehensive phylogenetic hypothesis for the order to date. Relationships at all ranks, such as the relationship of the early-diverging families, often reflect previous studies, but gene conflict is evident, and relationships previously found to be uncertain often remain so. Technical considerations for processing HTS data are also discussed.CONCLUSIONS: High- throughput sequencing and the Angiosperms353 probe kit are powerful tools for phylogenomic analysis, but better understanding of the genetic data available is required to identify genes and gene trees that account for likely incomplete lineage sorting and/or hybridization events.
U2 - 10.1002/ajb2.1699
DO - 10.1002/ajb2.1699
M3 - Article
SN - 0002-9122
VL - 108
SP - 1087
EP - 1111
JO - American Journal of Botany
JF - American Journal of Botany
IS - 7
ER -